About Scaffolds

3DBS produces two scaffolds, the Fibrilla Scafold™ and the Amazon Fibrilla Scaffold™ both developed using the electrospinning technique. In addition, we also work on the demand of our customers, being able to electrospun polymers with different degradation rates and fiber diameters, generating matrices with pore sizes for different cell types and applications.

The great differential of our Fibrillas Scaffolds™ is the possibility of incorporating bioactive molecules, like the essential oils of copaiba and citronella.

FIBRILLA SCAFFOLDS™ APPLICATION:

Key steps for skin implants applications:

1

Polymer solution

The first step is the preparation of a poly (lactic-co-glycolic acid) PLGA solution, a bioresorbable polymer, with copaiba oil-resin

2

Electrospinning

The polymer solution is placed in a syringe and the electrospun. The result is a deposition of fibrous matrix (or scaffold), formed by aligned or random filaments. The Fibrilla Scaffolds™ is sterilized with gamma radiation (γ).

3

Cell Culture

In an oven at 37 °C, cells of the patient responsible for the synthesis of collagen, called fibroblasts, are cultured on the Fibrilla Scaffold™. Cells grow, proliferate and differentiate

4

Cell Growth

The pore size of the Fibrilla Scaffolds™ matrix allows the migration and proliferation of fibroblasts, bonding to each other and growing into layers forming a three-dimensional (3D) structure. This process takes 20 to 30 days

5

Implant

Finally, the artificial skin (or dermal substitute) formed by the Fibrilla Scaffold™ and dermal cells is ready to be implanted in patients with severe skin lesions, such as third degree burns, ulcers and bedsores